Monday, 22 August 2016

Revisiting the Current Assays Associated to Host Immune Responses Against Tuberculosis Infection

As an ancient microbe, Mycobacterium tuberculosis (MTB) is an extremely successful pathogen. MTB causes more deaths worldwide now than at any previous time in history as the World Health Organization (WHO) estimates that approximately one-third of the world’s population (roughly 2 billion total) is infected with MTB. MTB is a major health threat, causing 9 million new infections and between 2 and 3 million deaths annually. Future prospects look bleak due to the increasing impact of HIV and drug resistance MTB strains (MDR) on the TB epidemic.

The clinical course of TB provides clues as to the mechanisms that underlie MTB’s success as a pathogen. First, MTB establishes infection with a small inoculum, suggesting that it inhibits innate immune responses. Second, it often persists throughout the life of the host, suggesting evasion of adaptive immunity. Third, the transmission of MTB from one host to another typically depends upon the formation of lung cavities in which aerosols are generated by coughing or sneezing.

Tuberculosis Infection
The mechanisms of lung cavitation are complex but include bystander damage of healthy tissue by the host cellular immune response. It is generally thought that granuloma formation is a means by which the host controls certain pathogens, most notably mycobacteria and fungal species. Granulomas form in animal models of TB and in human infection the scarred result of the granulomatous response to initial infection is sometimes observed as a calcified lesion abutting the pleural in a lower lobe of the lung, the “Ghon lesion”. Although the granuloma limits the extent of early infection, it is ultimately cell-mediated immunity involving T-cells that control MTB replication.


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