Detection of Classical Swine Fever Virus by a Surface Plasmon Resonance Assay

Classical swine fever disease, a major concern in the swine-related populations, is caused by classical swine fever virus (CSFV) which belongs to the same flavivirus family of other human pathogens, namely Dengue fever virus, Yellow fever virus, Japanese encephalitis virus, and Tick-borne encephalitis virus. CSFV is anenveloped RNA virus, with a diameter of 40-60 nm and consisting of 12.3 kb witha genome size poly protein coding for 3898 amino acids. 

CSFV causes classical swine fever (CSF) characterized by acute haemorrhagic thrombocytopenia and immunosuppression. In less virulent strains of CSFV long-term persistence and inherited disease to offspring can occur. Commonly CSFV antigens could be traced from crude or impure samples with low concentrations of viruses by fluorescent antibody and ELISA. For convenience Risatti and co-workers developed a portable real-time RT PCR assay for in situ CSFV detection. The development of multiplex real-time RT PCR (RT-MRT-PCR) offers dual purpose, CSFV detection and genotyping, and for the quantitative and also differential detection of wild-type viruses from C-strain of CSFV in vaccinated swine herds. Read more>>>>>>>>>>>>>