This
was a retrospective observational study in which the medical records of
patients of cryptococcal meningitis were analyzed in view of their
demographics, presenting complaints, HIV status, co -morbid conditions,
diagnostic tests, treatment and outcome. Suspected CSF specimens were subjected
to direct India ink examination, urease test and culture. Presumptive
identification as C. neoformans species complex was done by salient
physiological features manifested by the colonies growing on Saboraud Dextroseagar (SDA) at 37ºC, positive urease test and chocolate brown coloured
yeast-like colonies on niger seed agar medium.
They were confirmed by ID 32 C
carbon assimilation profiles, read and interpreted by the mini API system
(Biomerieux). In vitro antifungal susceptibility of all confirmed isolates was
determined by broth microdilution method for amphotericin B (AMB),
5-fluorocytosine (5FC), fluconazole, ketoconazole, voriconazole and
itraconazole. The mating types of strains were determined as described by Yan
et al. The strains were genotyped by PCR fingerprinting with the repetitive
oligonucleotide (GACA) 4 used as a single primer. Read more..............................
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